First report of the presence of Trypanosoma evansi in dogs from Paraguay applying molecular techniques

Surra is a disease caused by the hemoflagellate pathogen Trypanosoma evansi which affects wide variety of mammals. The only cases reported presence T. in Paraguay were identified samples from horses and capybaras they detected light microscopy 19th century. aim this study to report three autochthonous canine trypanosomiasis evansi, for first time country, using molecular techniques their application differential diagnosis trypanosomatids species. technique implemented was real-time PCR-HRM, amplifying fragment hsp70 gene, pair primers initially used discriminate Leishmania This on usage these detect through HRM analysis, allows the differentiation species simultaneously, making it an efficient tool diagnosis.